Premium
Influence of Liquid Manure on Soil Denitrifier Abundance, Denitrification, and Nitrous Oxide Emissions
Author(s) -
Miller M.N.,
Zebarth B.J.,
Dandie C.E.,
Burton D.L.,
Goyer C.,
Trevors J.T.
Publication year - 2009
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj2008.0059
Subject(s) - denitrification , nitrous oxide , chemistry , environmental chemistry , bacteria , abundance (ecology) , manure , relative species abundance , denitrifying bacteria , agronomy , zoology , nitrogen , biology , ecology , genetics , organic chemistry
The influence of liquid dairy and swine manure, soil water‐extractable C (WEC) and glucose on the amount of denitrification, the N 2 O molar ratio [N 2 O/(N 2 O + N 2 )], and the abundance of soil denitrifiers was investigated using repacked soil cores. Quantitative polymerase chain reaction was used to measure the abundance of the total bacteria (16S rRNA gene), two denitrifier guilds bearing the cnorB gene, and nosZ gene communities. The availability of both C and N oxides influenced the amount of denitrification and the N 2 O molar ratio for simple (glucose) and complex (liquid manure and WEC) C sources. A positive relationship ( R 2 = 0.77) was measured between respiration (cumulative CO 2 emissions) and total denitrification. A negative relationship ( R 2 = 0.70) was found between respiration and the N 2 O molar ratio, demonstrating that C availability in soil promotes the reduction of N 2 O to N 2 The abundance of the 16S rRNA and cnorB B ( Bosea/Bradyrhizobium/Ensifer spp.) gene bearing bacteria was not significantly affected by the addition of C. The abundance of cnorB P ( Pseudomonas mandelii and related species) gene bearing bacteria increased in response to glucose and manure addition, but only when also amended with NO 3 − Significant positive correlations were found between the abundance of the cnorB P gene bearing bacteria and respiration. The nosZ gene bearing bacteria significantly increased only in soil amended with liquid manures. No significant relationships were found between the abundance of denitrifiers and total denitrification, N 2 O emissions, or the N 2 O molar ratio. Changes in denitrifier community abundance appeared to reflect changes in C substrate availability and were uncoupled to denitrification activity.