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Phosphorus in Poultry Litter and Soil: Enzymatic and Nuclear Magnetic Resonance Characterization
Author(s) -
He Zhongqi,
Honeycutt C. Wayne,
Cade-Menun Barbara J.,
Senwo Zachary N.,
Tazisong Irenus A.
Publication year - 2008
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj2007.0407
Subject(s) - chemistry , hydrolysis , phosphorus , poultry litter , litter , soil water , enzyme , organic matter , nuclear chemistry , environmental chemistry , zoology , nutrient , biochemistry , agronomy , organic chemistry , biology , ecology
Knowledge of the P forms in poultry litter (PL) and their transformations in soil will help improve our understanding of the long‐term role of P in eutrophication. In this study, samples of PL and pasture soils with and without 20 yr of PL application were sequentially extracted to separate P into H 2 O, 0.5 mol L −1 NaHCO 3 , 0.1 mol L −1 NaOH, and 1 mol L −1 HCl fractions. After appropriate dilution and pH adjustment, the fractions were incubated in the presence of orthophosphate‐releasing enzymes. Cross‐examination of the solution 31 P nuclear magnetic resonance spectra of the enzymatically treated and untreated fractions revealed that the peaks of organic P (P o ) species of the enzymatically treated fractions became very weak or disappeared, confirming enzymatic hydrolysis of P o in the untreated fractions. Although the majority of P in the NaOH and HCl fractions of PL was in organic forms, these stable P o forms could be subjected to enzymatic hydrolysis after being applied to soil, an occurrence that was supported by the soil P data. Compared with soil without litter applied, 20 yr of PL application increased the pools of both labile and stable inorganic P in the soil; however, repeated application of PL did not lead to a significant accumulation of hydrolyzable P o in NaOH and HCl fractions, indicating that the stable P o must have been converted to other forms. The transformation of stable PL P o observed in this study could be an important mechanism for maintaining a balance between labile and immobile P in soils.