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A Rapid Method to Estimate Potentially Mineralizable Nitrogen in Soil
Author(s) -
Picone L. I.,
Cabrera M. L.,
Franzluebbers A. J.
Publication year - 2002
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj2002.1843
Subject(s) - loam , chemistry , nitrogen , reagent , soil test , environmental chemistry , biomass (ecology) , mineralogy , soil water , soil science , agronomy , environmental science , organic chemistry , biology
Rapid estimates of mineralizable N in soil are important for management decisions and soil quality assessments. We adapted and evaluated a rapid method based on measuring the gas pressure generated when soil is treated with Ca(ClO) 2 in a closed vessel. An experiment was conducted to determine the effects of reaction time, soil/reagent, and soil/water ratios on the gas pressure generated by the method. Based on this experiment, 5 g of soil, 5 mL of deionized water, 0.3 g Ca(ClO) 2 , and a reaction time of 25 min were selected as optimum conditions. The method was evaluated with 60 Cecil (fine, kaolinitic, thermic Typic Kanhapludults) sandy loam samples ranging in organic C from 4 to 16 g C kg −1 Nitrogen mineralized in 24 d and soil microbial biomass C (SMBC) were measured and related to the Ca(ClO) 2 method and to two other rapid methods, the flush of CO 2 during 3 d following rewetting of a dry soil, and the NH 4 –N extractable with hot 2 M KCl. The Ca(ClO) 2 method (mmol kg −1 ) was strongly correlated with net N mineralized in 24 d ( r = 0.77) and with microbial biomass C ( r = 0.90). The method was also correlated with the flush of CO 2 during 3 d following rewetting of dried soil ( r = 0.85) and with the NH 4 –N extractable with hot 2 M KCl (r = 0.86). These results indicate that the Ca(ClO) 2 method may be useful to make rapid estimates of mineralizable N and microbial biomass C in soil. Additional work is needed to investigate the nature of the compounds oxidized by the method.