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Characterization of the Hydrophobic Acid Fraction Isolated from a Wheat Straw Extract
Author(s) -
Grossl Paul R.,
Inskeep William P.
Publication year - 1996
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1996.03615995006000010025x
Subject(s) - chemistry , osmometer , amberlite , elemental analysis , fourier transform infrared spectroscopy , nuclear chemistry , magic angle spinning , size exclusion chromatography , straw , chemical structure , chromatography , nuclear magnetic resonance spectroscopy , organic chemistry , inorganic chemistry , chemical engineering , adsorption , engineering , enzyme
The hydrophobic acid portion (WSE‐FA) was isolated from a water‐soluble extract of wheat ( Triticum aestivum L.) straw and chemically characterized. This material was isolated and concentrated on a column containing Amberlite XAD‐8 resin. Chemical analysis of the WSE‐FA included elemental composition (C, O, H, N, S, P, and ash); titrametric evaluation of carboxylic acid and phenolic functional group content; molecular weight (MW) determination by vapor pressure osmometry (VPO); gel filtration and ultrafiltration; and UV‐visible, solid‐state cross‐polarization magic angle spinning 13 C nuclear magnetic resonance (NMR), and Fourier‐transform infrared spectroscopy. The MW of the WSE‐FA was approximately 600 daltons. The 13 C‐NMR spectrum, the low MW relative to other humic substances, high E 4 / E 6 ratio, and the low N content suggested that the WSE‐FA was comprised of small, aromatic phenolic acids, oxidized polyphenols with exposed carboxyl groups, and saccharides that were condensed during senescence.