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Enzymatic Coupling of 2,4‐Dichlorophenol to Stream Fulvic Acid in the Presence of Oxidoreductases
Author(s) -
Sarkar Jawed M.,
Bollag JeanMarc,
Malcolm Ronald L.
Publication year - 1988
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1988.03615995005200030017x
Subject(s) - chemistry , laccase , 2,4 dichlorophenol , fulvic acid , enzyme , environmental chemistry , peroxidase , pollutant , chromatography , soil water , humic acid , organic chemistry , ecology , fertilizer , biology , bacteria , genetics
The coupling of 14 C‐ring‐labelled 2,4‐dichlorophenol (2,4‐DCP) to stream fulvic acid was investigated in the presence of several oxidoreductases including tyrosinase, peroxidase, and laccases of Rhizoctonia praticola and Trametes versicolor . During 12‐h incubation of each of the oxidoreductases with 14 C‐2, 4‐DCP and stream fulvic acid, a substantial amount of the radioactivity was incorporated into fulvic acid. Chromatographic analyses indicated that although a large portion of the radioactivity remained in solution, no unbound 14 C‐2,4‐DCP was present in the supernatant. The effects of pH, temperature, concentration of fulvic acid, and concentration of enzyme on the coupling processes were studied. The results of this research provide evidence that the enzymatic coupling of certain xenobiotic pollutants to humic substances is an important natural process which must be considered in studies of the fate, reactivity, and persistence of these organic compounds in soils and stream waters.