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Extraction of Catalase From Soil
Author(s) -
PerezMateos M.,
GonzalezCarcedo S.,
Busto Nuñez M. D.
Publication year - 1988
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1988.03615995005200020018x
Subject(s) - pyrophosphate , extraction (chemistry) , chemistry , catalase , chromatography , centrifugation , phosphate , sodium , nuclear chemistry , enzyme , biochemistry , organic chemistry
Experimental conditions for extraction of extracellular catalase from soil were studied by using tetra‐sodium pyrophosphate as extractive solution. Mixture of soil with 0.01 M pyrophosphate in a 1:2 ratio (w/v), adjustment of pH to 7.0–7.3, 18 h of reciprocating agitation at 1.05 × g (60 rpm) and bacteriological filtration after centrifugation were the optimum conditions for extraction of soil fractions with catalase activity. Concentration and pH of pyrophosphate were the factors with the most influence on the extraction yields. Some aspects of pH variation in successive extractions of enzyme and differences between the use of extractive solutions at a same buffer pH or control of extraction pH are reported.