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Determination of FeEDDHA in Soils and Fertilizers by Anion Exchange Chromatography
Author(s) -
Barak Phillip,
Chen Yona
Publication year - 1987
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1987.03615995005100040010x
Subject(s) - chemistry , chromatography , ion chromatography , column chromatography , elution , high performance liquid chromatography , calibration curve , detection limit , ion exchange , analytical chemistry (journal) , ion , organic chemistry
The specific conditions for the quantitative analysis of FeEDDHA (ethylenedimine di‐ o ‐hydroxyphenylacetic acid) by high pressure liquid chromatography (HPLC) were studied and defined. Using a 3‐cm anion exchange column and 5 m M H 2 SO 4 + 0.01 m M Fe 2 (SO 4 ) 3 as an eluent, FeEDDHA was separated into the dl‐racemic and meso isomers. With the same column and 3 m M H 2 SO 4 + 50 m M Na 2 SO 4 + 0.01 m M Fe 2 (SO 4 ) 3 , FeEDDHA eluted as a single peak. Standard calibration curves for FeEDDHA were obtained with regression coefficients of 0.9999 at concentrations up to 0.25 m M . The coefficient of variation is 1.5%, or 1.8 µmol L −1 using a 50‐µL sample; the detection limit was 1.2 µmol L −1 . FeEDDHA was separated easily on anion exchange HPLC columns from other chelates such as CDTA, EDTA, and DTPA. The proposed method was used to determine FeEDDHA concentrations in water extracts of soils that were incubated with the compound. With variation of the eluent, separation of ferrated and unferrated EDDHA is possible.