Premium
Ethylene Biosynthesis in Soil: II. Kinetics and Thermodynamics in the Conversion of 1‐aminocyclopropane‐1‐carboxylic Acid to Ethylene
Author(s) -
Frankenberger W. T.,
Phelan Patrick J.
Publication year - 1985
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1985.03615995004900060018x
Subject(s) - soil water , ethylene , chemistry , enthalpy , kinetics , nuclear chemistry , activation energy , stereochemistry , organic chemistry , catalysis , biology , thermodynamics , ecology , physics , quantum mechanics
Kinetic analyses were performed to characterize the conversion of 1‐aminocyclopropane‐1‐carboxylic acid (ACC) to ethylene (C 2 H 4 ) in soils. Application of three linear transformations of the Michaelis‐Menten equation indicated that the K m values varied slightly among soils, but were similar in magnitude. By using the Lineweaver‐Burk plot, the K m values in three soils ranged from 2.7 to 6.1 (avg = 4.8) m M and V max values ranged from 3.9 to 16.5 mmol of C 2 H 4 released kg −1 soil 48 h −1 . The activation energy ( E a ) and enthalpy of activation (Δ H *) values, expressed in kJ mol −1 , ranged from 35.4 to 70.5 (avg = 49.4) and 32.8 to 67.9 (avg = 46.8), respectively. The Q 10 values within a temperature range of 5 to 50°C ranged from 1.52 to 2.60. A direct relationship was established between soil pH of 20 unbuffered surface soils and ACC enhanced‐C 2 H 4 production, but no such relationship was found with any other soil parameter tested including organic C, total N, CEC, and texture. Antibiotic treatments of soils revealed that both fungistatic and bacteriostatic agents were effective in inhibiting (25–82%) ACC derived‐C 2 H 4 production in soils indicating that both major groups are active C 2 H 4 producers.