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A New Plant Growth Technique for the Most‐Probable‐Number Estimation of Rhizobium
Author(s) -
Garvin S.,
Lindemann W. C.
Publication year - 1983
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1983.03615995004700030043x
Subject(s) - rhizobia , rhizobium , microbial inoculant , biology , inoculation , cone (formal languages) , plant growth , horticulture , botany , mathematics , agronomy , symbiosis , bacteria , genetics , algorithm
Abstract A new system for growing plants in the most‐probable‐number (MPN) estimation of Rhizobium is described. Beans, peanuts, and alfalfa were grown in small plastic cone‐tainers and inoculated with suspensions of rhizobia. The number of rhizobia in pure culture, inoculants, and soil was satisfactorily estimated using cone‐tainers for host plant growth. Most‐probable‐number counts of R. phaseoli were comparable in growth pouches and cone‐tainers. The cone‐tainer system saves bench space and costs less than other presently used techniques.