Premium
An Agar Plate Method for Rapid Screening of Rhizobium for Tolerance to Acid‐Aluminum Stress
Author(s) -
Ayanaba A.,
Asanuma S.,
Munns D. N.
Publication year - 1983
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1983.03615995004700020016x
Subject(s) - agar , agar plate , rhizobium , liquid medium , aluminium , chemistry , chromatography , biology , food science , bacteria , horticulture , inoculation , organic chemistry , genetics
Abstract An agar plate method has been developed for the rapid, preliminary screening of large numbers of rhizohia for tolerance to acidity and aluminum (acid‐Al). Media containing pH indicators are used, and distinguish true tolerance from cases where growth depends on a previous pH shift. Strains which were highly tolerant or sensitive to acid Al on agar behaved similarly in defined liquid media. Strains that formed dry, pinpoint colonies were more sensitive to acid‐Al than those that formed large, gummy colonies.