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Extraction of Phosphatase, Urease, Proteases, Organic Carbon, and Nitrogen from Soil
Author(s) -
Nannipieri Paolo,
Ceccanti Brunello,
Cervelli Stefano,
Matarese Emilio
Publication year - 1980
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1980.03615995004400050028x
Subject(s) - urease , casein , chemistry , proteases , extraction (chemistry) , phosphatase , incubation , enzyme , phosphate , biochemistry , chromatography , pyrophosphate , nitrogen , total organic carbon , environmental chemistry , organic chemistry
Extractions of three soils at different incubation times demonstrate that 0.14 M sodium pyrophosphate at pH 7.1 extracts phosphatase, urease, casein, and benzoylarginamide hydrolyzing proteases. Extraction yields of phosphatase and casein‐hydrolyzing proteases are remarkably high. The most efficient length of extraction depends both on the type of soil and on the selected enzyme. Correlation analyses of extracted enzymes and extracted C and organic N content generally show a significant correlation (p = 0.05 and 0.01).