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Activity of Immobilized Pronase in the Presence of Montmorillonite
Author(s) -
Griffith S. M.,
Thomas R. L.
Publication year - 1979
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1979.03615995004300060015x
Subject(s) - pronase , montmorillonite , chemistry , incubation , chromatography , enzyme , enzyme assay , biochemistry , trypsin , organic chemistry
The activity of soluble and immobilized pronase in the presence of montmorillonite was examined using a differential spectrophotometric technique over a 100‐sec incubation period. On the basis of equivalent amounts of enzyme protein, the immobile enzyme had only 28% of the activity of the soluble pronase in the absence of clay. However, in the presence of clay, the immobile pronase was much less inhibited, retaining an average of 75% of its activity while the soluble pronase retained only 31% of its activity. The differential spectrophotometric procedure was satisfactory for monitoring the enzyme action in the presence of suspended clay.