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Microbial Epimerization of Myo‐inositol to Chiro‐inositol in Soil
Author(s) -
L'Annunziata Michael F.,
I. Juan Gonzalez,
Olivares O. Luis A.
Publication year - 1977
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1977.03615995004100040024x
Subject(s) - loam , inositol , chemistry , metabolite , epimer , chromatography , biochemistry , biology , stereochemistry , soil water , ecology , receptor
14 Carbon‐(1,2,3,5/4,6)‐inositol ( 14 C‐myo‐inositol) was incorporated into an Andosol sandy loam soil and incubated for a 12‐day period at 70% field moisture capacity under sterile and nonsterile soil conditions. Comparison by thin‐layer chromatography of an isolated metabolite with an authentic standard and infrared spectrometry were used to identify the metabolite as 14 C‐(1,2,4/3,5,6)‐inositol ( 14 C‐chiroinositol) of unknown optical activity. The 14 C‐chiro‐inositol was identified in the biologically active soil. No epimerization of 14 C‐chiroinositol was observed in sterile soil. The abundance of the 14 C‐chiroinositol was approximately 4% relative to that of the parent compound, as determined by liquid scintillation counting of the two chromatographically separated compounds.