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A Centrifugal Filtration Method for Isolating Rhizocylinder Solution
Author(s) -
Soon Y. K.,
Miller M. H.
Publication year - 1977
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1977.03615995004100010039x
Subject(s) - loam , filtration (mathematics) , soil water , phosphate , salt (chemistry) , chemistry , phosphorus , salt solution , centrifugation , soil science , chromatography , environmental science , mathematics , statistics , organic chemistry
Abstract To measure the phosphate concentration in the soil solution adjacent to plant roots, a method of isolating the rhizocylinder solution was developed. A major factor in the choice of a method is that it should be rapid and should not alter the solution salt concentration during isolation. The centrifugal filtration method satisfies both conditions for bulk soil samples; it was subsequently applied to the rhizocylinder (roots plus adhering soil). Root sap leaking from corn ( Zea mays . L) roots during centrifugation introduced a significant error in the rhizocylinder solution phosphate concentration for a sandy loam with a low solution phosphorus concentration. For two medium‐textured soils with a higher solution P concentration, the error was negligible. The method is considered suitable for the latter soils.