Premium
Virulence of the Alnus Endophyte after In Vitro Cultivation
Author(s) -
Rogers R. D.,
Wollum A. G.
Publication year - 1974
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1974.03615995003800050020x
Subject(s) - endophyte , virulence , inoculation , biology , cycloheximide , alnus glutinosa , botany , microbiology and biotechnology , fungus , nitrogen fixation , horticulture , bacteria , cell culture , alder , biochemistry , genetics , gene
A technique has been devised for maintaining the virulence of the endophyte of Alnus glutinosa L. during in vitro cultivation. Essential to the technique are the inclusion of an ether extract of Alnus roots and cycloheximide and chloramphenicol to the artificial medium. Using the devised medium, endophyte virulence has been maintained for 14 days whereas in the absence of the ether extract and antibiotics, virulence was lost within 3 days. Nodules that are subsequently formed after inoculation of plants with the incubated endophyte are normal. Visible nodules were formed within 7 to 12 weeks after inoculation, a period which was comparable to the nodulation time of control plants inoculated with fresh crushed nodules. In addition, the nodules were capable of reducing acetylene which is indicative of nitrogen fixing activity. Also, during this study it was found that freeze‐drying nodules does not reduce the virulence of the nodule‐inducing endophyte.