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An Evaluation of Zonal Centrifugation as a Research Tool in Soil Science: I. Methodology
Author(s) -
Francis C. W.,
Bonner W. P.,
Tamura Tsuneo
Publication year - 1972
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1972.03615995003600020043x
Subject(s) - isopycnic , saturation (graph theory) , silicate , centrifugation , particle (ecology) , layer (electronics) , chemistry , mineralogy , analytical chemistry (journal) , soil science , materials science , chemical engineering , geology , chromatography , nanotechnology , mathematics , organic chemistry , oceanography , engineering , combinatorics
The methodology involving zonal centrifugation of clay sized mienrals through density gradients is presented. Various aspects of the procedure such as gradient solvents, formation of the gradient, dispersing agents, and methods of band removal are discussed. The parameters of centrifugal force, saturating cation, and particle size were evaluated as to their effect on time and isopycnic banding location within the gradient. It was concluded that an ultracentrifuga is required (a force field greater than 623 g) to band isopycnically clay particles of < 0.2 microns in diameter. The saturating cation influenced banding of 2:1 expandable layer silicates to a greater degree than 1:1 layer silicates, and the spatial relationshp of the expanding layer silicate associated with specific cation saturation played a larger role in banding than differences in atomic weight among the saturating catons.