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Biosynthesis of Sugars in Soils Incubated with 14 C Glucose and 14 C Dextran
Author(s) -
Oades J. M.,
Wagner G. H.
Publication year - 1971
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1971.03615995003500060021x
Subject(s) - incubation , monosaccharide , rhamnose , chemistry , arabinose , xylose , galactose , dextran , hydrolysis , sugar , chromatography , biochemistry , carbohydrate , soil water , mannose , biology , fermentation , ecology
Samples of soil were incubated for 28 days after the addition of 14 C‐labeled glucose or 14 C‐labeled dextran. Decomposition was monitored by measuring the release of CO 2 and determining its radioactivity throughout the incubation period. Soil samples were removed from the incubation at intervals, hydrolyzed to release individual monosaccharides, and the radioactivity of each sugar was measured. The 14 C from glucose or dextran was rapidly incorporated into galactose and mannose during the incubations; but xylose, arabinose, rhamnose and fucose embodied smaller amounts of 14 C or none at all. At the end of 28 days, 75% and 66% of the 14 C had been liberated as CO 2 from the glucose and dextran incubations, respectively. Of the residual 14 C in the soils after 28 days, about 6% from the glucose incubation and 15% from the dextran incubation, was present in saccharides and predominantly found in the hexoses. A portion of the dextran was apparently protected from decomposition during the incubation.