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The Occurrence of 2‐Methoxy‐1,4‐Naphthoquinone in Caribbean Vertisols
Author(s) -
Lambert E. N.,
Seaforth C. E.,
Ahmad N.
Publication year - 1971
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1971.03615995003500030037x
Subject(s) - pigment , naphthoquinone , chemistry , chromatography , solubility , vertisol , column chromatography , soil water , organic chemistry , biology , ecology
An orange colored pigment identified as 2‐methoxy‐1,4‐naphthoquinone was separated and identified from the dark colored 0 to 15‐cm layer of Caribbean Vertisols. Extraction of the pigment was carried out by shaking the soil (> 2 mm) in CHCl 3 (1:2) and decanting the supernatant liquid from which the pigment was purified by column chromatography and crystallization from ethanol. Eight kilograms of soil yielded 69 mg of the pigment. Identification of the pigment was determined using a combination of techniques including solubility tests, elemental analyses, melting point, ultraviolet, infrared, NMR, and mass spectroscopy. To the authors' knowledge, the isolation described herein is the first for a nonchlorophyllic pigment occurring in a chemically uncombined form in a tropical soil.