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Arylsulfatase Activity of Soils
Author(s) -
Tabatabai M. A.,
Bremner J. M.
Publication year - 1970
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1970.03615995003400020016x
Subject(s) - arylsulfatase , chemistry , soil water , chromatography , arylsulfatase a , hydrolysis , toluene , sulfate , enzyme , environmental chemistry , biochemistry , organic chemistry , environmental science , soil science
The detection of arylsulfatases (enzymes that hydrolyze aryl sulfates) in soils is reported, and a simple method of assaying soil arylsulfatase activity is described. This method involves colorimetric estimation of the p ‐nitrophenol released by arylsulfatase activity when soil is incubated with buffered (pH 5.8) potassium p ‐nitrophenyl sulfate solution and toluene at 37C for 1 hour, and it is rapid, sensitive, and precise. The procedure used to extract p ‐nitrophenol stops arylsulfatase activity, develops the yellow color used to estimate p ‐nitrophenol, and gives quantitative recovery of this compound. Studies of the properties of the arylsulfatases in soils are reported.