Premium
A Simple Technique for Preparing and Maintaining Sterile Soils for Plant Studies
Author(s) -
Bartlett R. J.,
Zelazny L. W.
Publication year - 1967
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1967.03615995003100030039x
Subject(s) - cooker , polyethylene , sterilization (economics) , soil water , detoxication , penetration (warfare) , propylene oxide , environmental science , materials science , pulp and paper industry , chemistry , composite material , soil science , organic chemistry , polymer , engineering , mechanical engineering , ethylene oxide , operations research , copolymer , monetary economics , economics , foreign exchange market , enzyme , foreign exchange
The proposed method depends upon the antimycotic activity of propylene oxide and the permeability of polyethylene. Inside a warmed pressure cooker, the epoxide diffuses rapidly through a plastic bag, sterilizing the moist soil contained. Release of pressure reverses diffusion. Detoxication is completed under vacuum at 45C. The bag then serves as a chamber for the storage of sterile soil or for the sterile culture of plants.