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Phytotoxic Substances Extracted from Soil
Author(s) -
Guenzi W. D.,
McCalla T. M.
Publication year - 1966
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1966.03615995003000020021x
Subject(s) - chemistry , acetone , column chromatography , chromatography , phloroglucinol , pyrophosphate , organic chemistry , enzyme
Abstract Phytotoxic substances were extracted from soil with 0.1 m Na 4 P 2 O 7 and acetone. The ether‐soluble substances from the pyrophosphate extract were separated into acidic, neutral, and basic fractions. Acidic materials exhibited the highest phytotoxicity, followed by basic materials and then by the neutral fraction. The acetone extract of soil was fractionated on an alumina column using a solvent elution series. A number of compounds were isolated by paper chromatography and the majority of these substances were either ninhydrin‐positive or acidic. Three phenolic acids were isolated and identified as vanillic, p ‐hydroxybenzoic, and protocatechuic. Ferulic, p ‐coumaric, p ‐hydroxybenzoic, vanillic, and syringic acids were extracted with 2 n NaOH from plowed and subtilled soil. Larger amounts of ferulic and p‐coumaric acids were found in subtilled soil as compared with the plowed soil. Only traces of these phenolic acids were found with 0.1 m sodium pyrophosphate, calcium oxide, 2 n HCl, and methanol extracts of soil.