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Soil Nitrogen Complexes: II. Electrophoretic Separation of Organic Components
Author(s) -
Waldron A. C.,
Mortensen J. L.
Publication year - 1961
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj1961.03615995002500010017x
Subject(s) - nitrogen , chemistry , hydrolysate , electrophoresis , organic matter , amino sugar , hydrolysis , chromatography , amino acid , sugar , nuclear chemistry , organic chemistry , biochemistry
Extracts of soil organic matter solubilized by 0.1 M Na 4 P 2 O 7 and hydrolyzed by 0.5 N NaOH, 6 N HCl, and 0.1 M Na 4 P 2 O 7 + 0.5 N NaOH were electrically desalted and subjected to continuous flow paper electrophoresis. Determination of organic matter, total nitrogen, α‐amino nitrogen and amino sugar in the collection tubes revealed the presence of nitrogen‐containing components having different electrophoretic mobilities. NaOH and HCl hydrolysates contained several nitrogenous components in which α‐amino nitrogen and amino sugars were detected. These hydrolysates also contained a brown‐colored component which contained most of the organic matter. The Na 4 P 2 O 7 extract, which exhibited considerable loss of organic nitrogen during desalting, contained one major component in which most of the nitrogen and brown material was contained. A fluorescent component was associated with the brown‐colored component in each extract.