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QTL Mapping of Charcoal Rot Resistance in PI 567562A Soybean Accession
Author(s) -
Silva Marcos P.,
Klepadlo Mariola,
Gbur Edward E.,
Pereira Andy,
Mason Richard E.,
Rupe John C.,
Bluhm Burt H.,
Wood Lisa,
Mozzoni Leandro A.,
Chen Pengyin
Publication year - 2019
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2018.02.0145
Subject(s) - biology , macrophomina phaseolina , quantitative trait locus , charcoal , population , plant disease resistance , horticulture , agronomy , botany , genetics , gene , materials science , demography , sociology , metallurgy
Charcoal rot of soybean [ Glycine max (L.) Merr.], caused by the soilborne fungus Macrophomina phaseolina (Tassi) Goid., has ranked among the most important soybean diseases in the United States. Disease management is typically done in a multifaceted approach through crop rotation, tillage, irrigation, and seed treatments aimed at minimizing damage caused by the pathogen. Development of genetic resistance to charcoal rot appears to be the most efficient strategy to control the disease; however, there are no reports of genetic regions associated with resistance to the disease. The objective of this study was to identify quantitative trait loci (QTLs) governing resistance to charcoal rot in soybean using a biparental population of PI 567562A (resistant) × PI 567437 (susceptible). A total of 140 F 2 –derived lines were genotyped with 5403 single nucleotide polymorphism (SNP) markers covering 20 chromosomes, of which 2283 were polymorphic. Resistance to charcoal rot was evaluated in the F 2:3 lines using the cut‐stem inoculation technique under greenhouse conditions. The QTL mapping analysis indicated one QTL on chromosome 15 explaining 29.4% of phenotypic variation, and two QTLs on chromosome 16 explaining 25.4 and 8.4% of phenotypic variation for resistance to M. phaseliona . To our knowledge, this is the first report of genomic regions harboring resistance to charcoal rot in soybean, and it may facilitate breeding and molecular engineering progress to combat charcoal rot disease in the future.