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Phylogenetic Analysis and Expression Profiles of Starch Phosphorylase Family Genes in Cassava under Diverse Environments
Author(s) -
Pei Jinli,
Zhang Shengkui,
Xia Zhiqiang,
Chen Xin,
Liu Chen,
Zhou Xincheng,
Ma Pingan,
Wang Wenquan
Publication year - 2018
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2017.10.0614
Subject(s) - biology , methyl jasmonate , starch , petiole (insect anatomy) , abscisic acid , gene , glycogen branching enzyme , gene family , phylogenetic tree , biochemistry , gene expression , botany , salicylic acid , abiotic stress , glycogen phosphorylase , genetics , enzyme , hymenoptera
Starch phosphorylase (Pho), especially the plastidial type, plays an important role in starch biosynthesis. The availability of a complete cassava ( Manihot esculenta Crantz) genome database allowed the identification of five Pho family members, which could be grouped into the plastidial type ( MePho1a , MePho1b , and MePho1c ) and cytosolic type ( MePho2 and MePho3 ) on the basis of phylogenetic analysis. Spatial gene expression analysis of various cassava tissues revealed that plastidial MePho1b had the highest transcriptional activity in root stele, whereas the cytosolic MePho2 was expressed with higher levels in leaves and petiole. Spatial expression analysis showed that MePho1b was strongly expressed in the tuber during root development process, especially at 70 and 180 d after planting, whereas MePho1a had highest expression in leaves at 180 d after planting. Expression of MePho1b and MePho2 could be induced by salt, drought, exogenous abscisic acid, methyl jasmonate, and salicylic acid signal in the leaves of abiotic stress‐treated plantlets. In addition, the expression profiles of MePho1b and starch branching enzyme gene member MeSBE2.2 exhibited significant correlation under diverse stress environments, indicating that they may have coordinated functions.