Premium
Re‐Engineering of the Pm21 Transfer from Haynaldia villosa to Bread Wheat by Induced Homoeologous Recombination
Author(s) -
Lukaszewski Adam J.,
Cowger Christina
Publication year - 2017
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2017.03.0192
Subject(s) - powdery mildew , biology , blumeria graminis , villosa , genetics , grain yield , recombination , locus (genetics) , agronomy , gene , plant disease resistance
Blumeria graminis f. sp. tritici , the cause of powdery mildew, can generate serious grain yield losses in wheat ( Triticum sp.). To expand the range of resistance genes freely available to wheat breeders, a Haynaldia villosa (L.) Schur. (syn. Dapsypyrum villosum L.)‐derived gene, Pm21 , was transferred to chromosome 6AS of wheat by homoeologous recombination. The transfer showed that the genetic location of the locus was different from that suggested by an earlier transfer by irradiation. Wheat lines with two small intercalary inserts of H. villosa chromatin with Pm21 were tested with a range of powdery mildew isolates and found to be completely resistant (infection score of 0).