Premium
Molecular Genetic Analysis of a Novel Recessive White Flower Gene in Wild Soybean
Author(s) -
Kim Ji Hong,
Bae Dong Nyuk,
Park SooKwon,
Jeong Namhee,
Lee Kwanghee,
Kang Hoyoung,
Kang SungTaeg,
Moon JungKyung,
Park Euiho,
Jeong SoonChun
Publication year - 2017
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2017.03.0163
Subject(s) - biology , locus (genetics) , glycine soja , genetics , allele , white (mutation) , population , gene , genetic analysis , botany , glycine , demography , amino acid , sociology
Cultivated soybean [ Glycine max (L.) Merr.] flowers are either white or purple, whereas nearly all wild soybean ( G. soja Sieb. and Zucc.) accessions have purple flowers. As a result, the soybean flower color phenotype has attracted the attention of plant breeders, biochemists, and population geneticists. Here, we report genetic mapping of a novel recessive white flower gene in YWS415, a wild soybean accession collected in Korea. The W1 locus, which is the major locus determining purple ( W1 ) or white ( w1 ) flower color, encodes flavonoid 3′ 5′‐hydroxylase in YWS415, which was more similar to the common W1 ‐type sequences than w1 ‐type sequences. Subsequent mapping results using an F 2 population from a cross between the ‘Hwangkeum’ (purple flower) and YWS415 variants indicated that the white flower color was controlled by a recessive allele at the W4 locus that encodes dihydroflavonol‐4‐reductase 2 (DFR2). The w4 allele arose through insertion of a 5584‐bp nonautonomous transposon member of soybean GM_CACTA_33 family into the second intron of DFR2 . It was designated as GM_CACTA_33_w4 . Results from this study will enhance our understanding of soybean white flower inheritance and facilitate marker‐assisted selection for the antioxidative anthocyanins in soybeans.