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Engineered Versions of the Wheat Lr62 Translocation
Author(s) -
Somo Mohamed,
Pirseyedi Seyed Mostafa,
Cai Xiwen,
Marais Francois
Publication year - 2017
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2016.08.0683
Subject(s) - biology , chromosomal translocation , locus (genetics) , genetics , chromosome , chromosome pairing , telomere , gene , chromosomal rearrangement , karyotype
Leaf rust resistance gene Lr62 was transferred from Aegilops neglecta Req. ex Bertol. to chromosome 6A of common wheat ( Triticum aestivum L.) in 2008. After homoeologous chromosome pairing induction, smaller translocations that retained Lr62 were derived from it. This study compared the original translocation and the four apparently shortest recombinants to determine which would be the best suited for breeding. The translocations were analyzed using chromosome 6A and 6B simple sequence repeat (SSR) markers and genomic in situ hybridization. Recombined translocations 133, 135, and 136 were found to occur at the 6AS telomere. In addition to the wheat Xdupw217 aest SSR locus on chromosome arm 6BS, the three recombinants also expressed an Ae. neglecta –derived homoeo‐locus ( Xdupw217 negl ) that could be used to detect the presence of the Ae. neglecta chromosomal segment. The Lr62 ‐129 translocation was relocated to 6BS in an allosyndetic recombination event that replaced the Xdupw217 aest locus with Xdupw217 negl . Lr62 ‐129 retained the least Ae. neglecta chromatin and is therefore recommended for use in wheat breeding.