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Ribosomal RNA Internal Transcribed Regions Identify Possible Misidentification or Mislabeling among Trifolium (Clover) Specimens from Germplasm Collections
Author(s) -
Jreisat Christina S.,
Laten Howard M.
Publication year - 2017
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2016.07.0626
Subject(s) - internal transcribed spacer , biology , germplasm , ribosomal dna , ribosomal rna , spacer dna , identification (biology) , ribosomal intergenic spacer analysis , hybrid , genetics , botany , gene , phylogenetics
Accurate identification and labeling of plant germplasm is essential for creating new hybrids with enhanced agronomic characteristics and for basic genetic and evolutionary studies. Expert collectors and seed banks do their best to attain high levels of accuracy and reliability, but errors are inevitable. During the course of a study of clover ( Trifolium ) transposable elements, we obtained DNA sequence results that led us to question the species designation of some accessions. By sequencing the internal transcribed spacer regions of the nuclear ribosomal RNA gene, we show that 4 of the 13 specimens we obtained appear to have been mislabeled or misidentified.