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Use of Hybrid Rice and the Proliferation of Weedy Rice: A Case in Jiangsu, China
Author(s) -
Zhang Banghua,
Dong Erjia,
Zhang Hu,
Shi Zhihua,
Song Xiaoling,
Qiang Sheng,
Dai Weimin
Publication year - 2016
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2015.04.0210
Subject(s) - weedy rice , biology , oryza rufipogon , cultivar , oryza sativa , genetically modified rice , gene , gene pool , molecular marker , polymerase chain reaction , botany , microbiology and biotechnology , horticulture , genetics , genetically modified crops , transgene , population , genetic diversity , demography , sociology
Jiangsu Province, located between northern China and southern China, is a typically mixed area of conventional and three‐line hybrid rice without Oryza rufipogon Griff. and Oryza nivara S. D. Sharma & Shastry. In the recent decade, occurrence of weedy rice rapidly increased year by year since it was found and reported from 2000. The recent cloning of the CMS‐WA gene (wild abortive cytoplasmic male sterility) makes it possible to design a marker specific for the gene for testing large quantities of weedy rice samples in a single assay by polymerase chain reaction (PCR). Through performing a BLAST search in the National Center for Biotechnology Information (NCBI) database using the sequence of the CMS‐WA gene cloned from ZS97A as a reference, the major difference between three‐line hybrid rice and conventional rice cultivars was found in the 287 to 439 bp region of the CMS‐WA gene. A specific marker, WA7, was designed and evaluated for this region of the CMS‐WA gene. This marker, in combination with RED4, a specific marker for the Rc red pericarp gene, was used to distinguish weedy rice, three‐line hybrid rice, and conventional rice in a single PCR assay. A total of 1232 accessions of weedy rice collected from 63 locations in Jiangsu Province, China, were evaluated using the red pericarp and CMS‐WA DNA markers. Only four accessions from three locations were found positive for the CMS‐WA gene, accounting for just 4.76 and 0.32% of all locations and accessions, respectively. These results indicate that the three‐line hybrid is likely a source but not the major source of weedy rice in Jiangsu Province.