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Quantitative Trait Loci Associated with Tolerance to Seed Cracking under Chilling Temperatures in Soybean
Author(s) -
Yamaguchi Naoya,
TaguchiShiobara Fumio,
Sayama Takashi,
Miyoshi Tomoaki,
Kawasaki Michio,
Ishimoto Masao,
Senda Mineo
Publication year - 2015
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2015.02.0081
Subject(s) - biology , quantitative trait locus , cultivar , phytotron , marker assisted selection , allele , population , agronomy , horticulture , inbred strain , genetics , gene , demography , sociology
In Hokkaido, northern Japan, soybean [ Glycine max (L.) Merr.] crops are damaged by cold weather. Chilling temperatures result in the appearance of cracked seeds (CSs) in soybean crops, especially those grown in eastern and northern Hokkaido. The seed coats of CSs are severely split on the dorsal side, and the cotyledons are exposed and frequently separated. The occurrence of CSs causes unstable production because these seeds have no commodity value. The aims of this study were to identify quantitative trait loci (QTL) associated with CS tolerance. In a previous study, a selection method for CS tolerance using a phytotron was developed. A recombinant inbred line (RIL) population was bred from a cross between a CS‐tolerant breeding line, Toiku 248 (T248), and a CS‐sensitive cultivar, Yukihomare (YH). Two stable QTLs, qCS8‐1 and qCS11‐1 , were identified by QTL analysis using the cracked‐seed index (CSI), the arcsine transformed CS ratio. Two pairs of near‐isogenic lines (NILs) were bred for the qCS8‐1 and qCS11‐1 loci to validate the effects of the QTLs. The CSIs of the NILs with the T248 allele were significantly lower than those with the YH allele at both the qCS8‐1 and qCS11‐1 loci. The T248 alleles at qCS8‐1 and qCS11‐1 had no negative influence on flowering time or other agronomic traits in the NILs. Our results suggest that marker‐assisted selection (MAS) for qCS8‐1 and qCS11‐1 would be effective for the breeding of CS‐tolerant cultivars.