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Identification and Analysis of Zbs1, a Dominant Male‐Sterile Mutant of Naked Oat ( Avena nuda L.)
Author(s) -
Yang Cai,
Zhou Haitao,
Shi Xiao,
Zhang Xinjun,
Li Tianliang,
Yang Xiaohong,
Zhang Qian,
Lu Tiegang
Publication year - 2016
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2014.07.0525
Subject(s) - biology , avena , mutant , microspore , stamen , callose , botany , staining , inflorescence , pollen , genetics , gene
A dominant male‐sterile mutant of naked oat ( Avena nuda L. ‘Zbs1’) was identified in a plant breeding program for accession A. nuda ‘Pin5’, a widely cultivated naked oat variety in northwest China. No significant differences were detected between Zbs1 and wild‐type plants during the vegetative growth stage. However, at the reproductive stage Zbs1 was significantly different from wild‐type in terms of spikelet morphology and anther development. Iodine‐potassium iodide staining, fluorescein diacetate staining, and germination tests showed that more than 95% of the Zbs1 pollen grains were not viable. Aniline blue staining showed that callose was absent or degraded during the dyad and tetrad stages of microsporogenesis. Furthermore, genetic and histological analyses revealed that Zbs1 is a dominant male‐sterile mutant with a stable, heritable phenotype that is not affected by cytoplasmic or environmental factors (e.g., location and photoperiod). These results indicate that Zbs1 is a novel, stable male‐sterile mutant of naked oat that can be used in breeding programs to facilitate recurrent selection and multi‐crossing schemes.