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Characterization of Alkali Stress‐Responsive Genes of the CIPK Family in Sweet Sorghum [ Sorghum bicolor (L.) Moench]
Author(s) -
Guo Ming,
Liu Qing,
Yu Hui,
Zhou Tingting,
Zou Jing,
Zhang Hui,
Bian Mingdi,
Liu Xuanming
Publication year - 2015
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2013.08.0520
Subject(s) - biology , gene , sweet sorghum , sorghum , gene family , gene expression , kinase , signal transduction , genetics , botany , biochemistry , microbiology and biotechnology , ecology
Calcineurin B–like protein (CBL) interacting protein kinases (CIPKs) are critical components in various stress signal transduction pathways. In previous studies, 32 putative CIPK genes ( SbCIPK s) were identified in sweet sorghum [ Sorghum bicolor (L.) Moench]. In the present study, a conserved sucrose nonfermenting 1 (SNF1)–like kinase domain in the N terminus and a NAF motif (a 24‐amino‐acid domain with the conserved amino acids N, A, and F required for the CBL–CIPK interaction) in C terminus were identified in all SbCIPK s using sequence analysis. Stress‐ and light‐responsive cis ‐elements were also identified in the promoter regions of sorghum CIPKs. The real‐time polymerase chain reaction analysis revealed different expression patterns among the 32 SbCIPK s under sodium carbonate (Na 2 CO 3 ) stress in sweet sorghum seedlings. Based on the expression patterns, six SbCIPK s were upregulated >10‐fold at the transcriptional level. Interestingly, these six CIPKs showed leaf‐specific expression under normal growth conditions, suggesting a relationship between various regulations in leaf function and alkali stress responses in sweet sorghum. Systemic yeast two‐hybrid assay demonstrated the specific interaction between multiple SbCIPK s and SbCBL s. Thus, this study provides the first characterization of the role of the CIPK family proteins in response to alkali stress.