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TILLING in European Rice: Hunting Mutations for Crop Improvement
Author(s) -
Casella Laura,
Greco Raffaella,
Bruschi Gianluca,
Wozniak Barbara,
Dreni Ludovico,
Kater Martin,
Cavigiolo Stefano,
Lupotto Elisabetta,
Piffanelli Pietro
Publication year - 2013
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2012.12.0693
Subject(s) - tilling , biology , oryza sativa , germplasm , population , methane sulfonate , crop , microbiology and biotechnology , genetics , agronomy , gene , mutant , demography , sociology
Targeting induced local lesions in genomes (TILLING) is a powerful technique that exploits variation induced by classical mutagenesis for gene discovery and functional studies as well as crop improvement. Here we describe the development and validation of the first rice ( Oryza sativa L.) TILLING platform of a European temperate japonica accession. A total of 1860 M 2 ethyl methane sulfonate (EMS)‐mutagenized lines were generated in the variety ‘Volano’, one of the most widely cultivated European rice varieties representative of the traditional Italian high quality rice. The validation of the TILLING population was performed by screening the M 2 lines for variation in four target genes of relevance for the improvement of Volano ( SD1 , Hd1 , SNAC1 , and BADH2 , involved in determining plant height, flowering time, drought tolerance, and aroma, respectively). Two independent mutations identified in the Green Revolution gene SD1 ( semidwarf 1 ) were demonstrated to have a significant phenotypic effect, resulting in semidwarf progenies with an average height reduction of 21% in the plants carrying the mutant allele in the homozygous state. The density of one mutation every 373 kb estimated in the Volano TILLING population was comparable to that previously obtained in rice EMS‐mutagenized populations and confirmed the effectiveness of this approach for targeted improvement of European temperate rice germplasm. Besides the validation of the TILLING platform, this work also provides genetic material that can be directly exploited for the improvement of the Volano variety.