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Development of Simple Sequence Repeat Markers and the Analysis of Genetic Diversity and Ploidy Level in a Centipedegrass Collection
Author(s) -
HarrisShultz Karen R.,
MillaLewis Susana R.,
Zuleta M. Carolina,
Schwartz Brian M.,
Hanna Wayne W.,
Brady Jeff A.
Publication year - 2012
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2011.03.0151
Subject(s) - biology , upgma , genetic diversity , germplasm , ploidy , genetics , accession number (library science) , botany , genetic variation , gene , genbank , population , demography , sociology
Little is known about the genetic variability of centipedegrass [ Eremochloa ophiuroides (Munro) Hack.] and few genetic tools have been available for this species. In this study, 69 unique Eremochloa sequences were generated by using a compound simple sequence repeat (SSR)‐based cloning method. Twenty‐nine of these clones contained an internal SSR and 30 specific primer pairs were developed that produced suitable amplification. The level of genetic diversity was assessed using 55 centipedegrass accessions and one Eremochloa zeylanica Hack. accession using primer pairs developed from the compound SSR‐based cloning technique. Twenty‐four polymorphic fragments could be scored and unweighted pair‐group method using arithmetic averages (UPGMA) cluster analysis showed that the Eremochloa accessions clustered into two groups: a large cluster of E. ophiuroides accessions and a group containing the single E. zeylanica accession. Principle coordinate analysis further divided the centipedegrass accessions into three groups. Ploidy analysis revealed all centipedegrass accessions were diploid while the single E. zeylanica accession was found to be a putative tetraploid. Furthermore, many of these markers can be used for other species belonging to the subfamily Panicoideae. The division of the centipedegrass accessions into groups and analysis of ploidy level provides information that will aid in the effective use of this germplasm in breeding programs.