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Characterization of a New Green‐Revertible Albino Mutant in Rice
Author(s) -
Wu Liquan,
Li Ruiqing,
Shu Qingyao,
Zhao Haijun,
Wu Dianxing,
Li Juan,
Wang Rongfu
Publication year - 2011
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2011.01.0008
Subject(s) - biology , mutant , oryza sativa , chlorophyll , chloroplast , indel , botany , wild type , phenotype , gene , horticulture , microbiology and biotechnology , genetics , genotype , single nucleotide polymorphism
The rice ( Oryza sativa L.) mutant G 9 , isolated from ‘Guangzhan63S’ exposed to 60 Co γ irradiation in combination with in vitro tissue culture, exhibits albino and growth stage‐dependent green‐revertible phenotypes. The albino phenotype was observed in the mutant seedlings up to the fourth leaf (L4) stage, and then the seedlings entered into a transit period when old and newly extended leaves gradually revert to partial green. Finally, the leaves turn into full green at the sixth leaf (L6) stage. Chlorophyll abundance in the mutant seedlings at the second leaf (L2) stage was quite low and increased to about 1/4 of wild type Guangzhan63S at the L4 stage. At the L6 stage, the levels of chlorophyll in G 9 were almost identical to that in Guangzhan63S. Ultra‐microstructure analysis showed that the G 9 mutant had no chloroplast at the L2 stage and fewer and smaller undifferentiated plastids at the L4 stage than Guangzhan63S at the same stage; no significant difference in chloroplast structure was found between G 9 and Guangzhan63S at the L6 stage. We also performed the simple sequence repeat (SSR) haplotype analysis to map the gene(s) responsible for the albino phenotype. Our data revealed that the green‐revertible albino trait of G 9 was controlled by a single recessive nucleic gene, named clb GZ , located between the indel markers of IDJ6 (0.16 cM) and IDJ12 (0.25 cM) on the rice chromosome 11.