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Rumen‐Degradable Protein in Roll‐Conditioned or Macerated Legume Hays and Silages Estimated by In Situ Kinetics vs. Alternative Methods
Author(s) -
Grabber J. H.,
Coblentz W. K.,
Broderick G. A.
Publication year - 2011
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2010.10.0566
Subject(s) - biology , rumen , forage , legume , in situ , red clover , protease , digestion (alchemy) , incubation , medicago sativa , ruminant , zoology , food science , botany , agronomy , biochemistry , fermentation , pasture , enzyme , chromatography , chemistry , organic chemistry
Alternatives to the in situ method for estimating rumen‐degradable protein (RDP) in diverse forage legumes should be validated. In this study, RDP in roll‐conditioned or macerated silages and hays of Medicago, Lotus, and Trifolium species with differing polyphenol compositions were estimated from in situ degradation kinetics, a rumen microbial inhibitor in vitro assay, a 10‐h in situ incubation, the Cornell Net Carbohydrate and Protein System, and a 16‐h in vitro Streptomyces griseus protease digestion. Forage species, conditioning, and conservation methods influenced RDP estimates. In situ kinetic RDP could be ranked or in some cases accurately predicted by other RDP methods, but the protease method performed poorly for macerated forages.