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Identification of Tobacco Haploids on the Basis of Transgenic Overexpression of PAP1 from Arabidopsis thaliana
Author(s) -
Lewis Ramsey S.,
Rose Cara
Publication year - 2011
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2010.09.0546
Subject(s) - biology , nicotiana tabacum , seedling , ploidy , botany , parthenogenesis , cultivar , arabidopsis , transgene , quantitative trait locus , genetics , gene , embryo , mutant
Haploid plants have utility in plant breeding for several purposes. In tobacco ( Nicotiana tabacum L.) gynogenic haploids can be produced from seed due to parthenogenesis. For practical use, a system is needed to identify infrequent haploid plants at the seed or seedling stage. Interspecific hybridization with N. africana is presently used to isolate gynogenic tobacco haploids because a suitable dominant seedling marker does not currently exist for N. tabacum . Here we investigated the utility of a purple seedling trait conferred by overexpression of the Arabidopsis gene, PAP1 , to identify gynogenic haploids produced from seed. Two tobacco cultivars were crossed as females with a genetic stock homozygous at two 35S : PAP1 transgene loci. Gynogenic haploids were recognized as green seedlings among purple F 1 hybrid seedlings. The average frequency of gynogenic haploidy using this system was f = 0.00027. For comparison, the same cultivars were also hybridized as females with N. africana . The average frequency of gynogenic haploidy using this system was approximately seven times higher than that observed for the 35S : PAP1 system. Having a dominant seedling marker for N. tabacum may permit development of genetic stocks that contribute to an increased predisposition for haploid formation via parthenogenesis. In addition, the 35S : PAP1 genetic marker may have utility for identifying androgenic haploids from seed for the purpose of rapidly generating alloplasmic lines of tobacco.