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Molecular and Cytogenetic Characterization of Wheat Introgression Lines Carrying the Stem Rust Resistance Gene Sr39
Author(s) -
Yu Guo Tai,
Zhang Qijun,
Klindworth Daryl L.,
Friesen Timothy L.,
Knox Ron,
Jin Yue,
Zhong Shaobin,
Cai Xiwen,
Xu Steven S.
Publication year - 2010
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2009.10.0633
Subject(s) - stem rust , biology , chromosomal translocation , chromosome , genetics , common wheat , puccinia , chromatin , gene , botany , mildew
The stem rust ( Puccinia graminis Pers.:Pers. f.sp. tritici Eriks. and Henn.) resistance gene Sr39 , which confers resistance to TTKSK (Ug99), has been incorporated into the wheat ( Triticum aestivum L.) genome from Aegilops speltoides in the form of a chromosome translocation but it has not been deployed into adapted cultivars. In this study, we characterized translocation lines carrying Sr39 in four different wheat backgrounds with fluorescent genomic in situ hybridization (GISH) and simple sequence repeat (SSR) markers. The results indicated that RL5711 and RL6082 had translocation chromosomes of comparable structure. The translocation chromosome in PI 600683 has lost Ae. speltoides chromatin in the telomeric end of the 2S long arm. Six translocation lines derived from the cross PI 600683/3*HY438 had translocation chromosomes of comparable structure to the one found in PI 600683. However, one line (P9714‐AM03C51), showed a substantial reduction in Ae. speltoides chromatin in the short arm of the translocated chromosome. The study demonstrated that it is apparently feasible to shorten Ae. speltoides chromatin in some wheat‐ Ae. speltoides translocation lines. These results and the identification of diagnostic SSR markers will be useful in guiding chromosome manipulation efforts to further shorten the Ae. speltoides chromosome segments in these materials. Greenhouse inoculation of translocation lines with stem rust indicated that the Sr39 gene conditions resistance to at least seven stem rust races.