Premium
Genetic Variability of Cultivated Rhizoma Peanut
Author(s) -
Maas Andrea L.,
Anderson William F.,
Quesenberry Kenneth H.
Publication year - 2010
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2009.10.0583
Subject(s) - biology , germplasm , amplified fragment length polymorphism , arachis , cultivar , genetic diversity , genetic variability , population , genetic distance , genetic variation , botany , genetic drift , genetic marker , horticulture , genotype , genetics , demography , sociology , gene
Rhizoma peanut (RP; Arachis glabrata Benth.) is a vegetatively propagated tropical legume that combines high forage nutritive value and long‐term persistence under a wide range of grazing and harvested hay systems. The objectives of this study were to measure the genetic relatedness among 15 RP accessions and assess purity of accessions maintained at different locations. A total of 22 RP clones were tested and included eight duplicate accessions sourced from the national Arachis collections and germplasm collections from Florida, Texas, and Georgia. Fourteen amplified fragment length polymorphism (AFLP) primer combinations produced a total of 951 bands, with an average of 67.92 ± 6.56 bands per primer combination. Analysis by unweighted pair group mean algorithm found genetic similarity coefficients (GSAs) that ranged from 0.21 to 1.0. STRUCTURE analysis found minimal population structure or admixture for the RP lines in this study. The duplicated accessions showed a range of genetic distances from no genetic drift (GSA 1.0) to a high degree of drift (GSA 0.28). Overall, AFLP markers provided sufficient polymorphism to successfully differentiate RP clones and determine when genetic drift occurred. The AFLPs substantiated genetic dissimilarity of newly released cultivars from standard accessions. Results also indicated the potential for genetic drift occurring from spontaneous crossing among field‐grown accessions, which could lead to selection of improved cultivars.