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Genetic Diversity of Common Carpetgrass Revealed by Amplified Fragment Length Polymorphism Markers
Author(s) -
Wang Zan,
Kenworthy Kevin E.,
Wu Yanqi
Publication year - 2010
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2009.08.0472
Subject(s) - amplified fragment length polymorphism , biology , genetic diversity , germplasm , dendrogram , genetic distance , genetic marker , genetic variation , genetics , botany , gene , population , demography , sociology
Common carpetgrass [ Axonopus fissifolius (Raddi) Kuhlm.] is a perennial, warm‐season grass. Its low maintenance attributes make it a desirable turfgrass species for use in the southeastern United States. Information on the extent of genetic diversity at the molecular level for common carpetgrass germplasm is limited. Accordingly, the objectives of this study were to assess the genetic diversity and relatedness among 59 common carpetgrass accessions, including 48 accessions collected from the southeastern United States, one from Hawaii, one from the NPGS, and another nine derived from commercial seed (seed‐derived) using fluorescence‐labeled amplified fragment length polymorphism (AFLP) markers. Twenty selected AFLP primer combinations generated a total of 1046 amplification bands, of which 469 (44.1%) were polymorphic. Genetic similarity coefficients among accessions ranged from 0.807 to 0.980. A low level of genetic diversity (Shannon's information index = 0.24, gene diversity = 0.16) was observed for this collection. Naturalized accessions had relatively higher genetic diversity than seed‐derived accessions. Accessions from both sources were randomly distributed throughout the dendrogram with no definite geographic or source patterns. More comprehensive sampling throughout its center of origin is needed to increase the genetic diversity of common carpetgrass for breeding.