Premium
Molecular Mapping of Wheat Leaf Rust Resistance Gene Lr42
Author(s) -
Sun Xiaochun,
Bai Guihua,
Carver Brett F.,
Bowden Robert
Publication year - 2010
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2009.01.0049
Subject(s) - biology , bulked segregant analysis , aegilops tauschii , rust (programming language) , germplasm , marker assisted selection , genetic marker , powdery mildew , gene mapping , plant disease resistance , common wheat , wheat leaf rust , molecular marker , genetics , population , cultivar , sequence tagged site , botany , gene , chromosome , demography , sociology , virulence , computer science , programming language
Leaf rust, caused by Puccinia triticina Eriks., is an important foliar disease of wheat ( Triticum aestivum L.) worldwide. Leaf rust resistance gene Lr42 from Aegilops tauschii Coss. has been used as a source of rust resistance in breeding programs. To identify molecular markers closely linked to Lr42 , a segregating population of near‐isogenic lines contrasting for the presence of Lr42 was developed in the hard winter wheat cultivar Century background and evaluated for rust infection type at both seedling and adult‐plant stages. Simple sequence repeat (SSR) markers were screened using bulked‐segregant analysis. Two markers closely linked to Lr42 were identified on chromosome 1DS. The closest marker, Xwmc432 , is about 0.8 cM from Lr42 . Physical mapping of both SSR markers using Chinese Spring nullitetrasomic and ditelosomic genetic stocks confirmed that the markers linked to Lr42 were on 1DS. Markers for Lr42 were highly polymorphic between parents and among a diverse set of wheat germplasm collected from several countries, indicating that these markers are useful for marker‐assisted selection for Lr42 .