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Mode of Reproduction of Colchicine‐Induced Paspalum plicatulum Tetraploids
Author(s) -
Sartor María E.,
Quarin Camilo L.,
Espinoza Francisco
Publication year - 2009
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2008.05.0270
Subject(s) - apomixis , paspalum , biology , ploidy , meiosis , botany , sexual reproduction , parthenogenesis , bivalent (engine) , genetics , gene , embryo , chemistry , organic chemistry , metal
Paspalum plicatulum Michx. is a wild forage grass species. The common races are tetraploid and apomictic, while sexual diploid representatives have been reported sporadically. Objectives of this study were to induce sexual 4 x individuals from sexual diploids, determine the capacity for hybridization with other apomictic 4 x species closely related with P. plicatulum , and thus create a tetraploid sexual material cross compatible with apomictic 4 x species of the Plicatula group of Paspalum . Two induced tetraploid plants were recovered from germinating 2 x seeds treated for 24 h with colchicine. Bivalent and quadrivalent chromosomes were the most common association at meiosis in these autotetraploids. Embryological analysis and progeny tests using molecular markers revealed that both induced tetraploids reproduced sexually. Single‐seed screening by flow cytometry confirmed full sexual reproduction. These plants retained the high self‐incompatibility system of the diploids, but set seed after reciprocal crosses and when crossed with pollen of apomictic 4 x P. guenoarum Arechav. of the Plicatula group. Both sexual 4 x plants constitute the foundational material for plant improvement through gene exchange and selection in apomictic 4 x P. plicatulum and possibly in several apomictic species of the Plicatula group.