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Molecular Identification of Saccharum spp. × Erianthus fulvus Hybrids Using Sequence‐Characterized Amplified Region Markers
Author(s) -
Wang XianHong,
Yang QingHui,
Li FuSheng,
He LiLian,
He ShunChang
Publication year - 2009
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2008.04.0241
Subject(s) - rapd , biology , hybrid , saccharum , primer (cosmetics) , polymerase chain reaction , genetic marker , genetics , molecular marker , cultivar , botany , gene , genetic diversity , population , chemistry , demography , organic chemistry , sociology
Identification of hybrids derived from crosses between cultivated sugarcane ( Saccharum spp.) and its wild relatives, including Erianthus fulvus Nees ex Hack., is important for the development of new sugarcane cultivars. The aim of the present study was to identify true hybrids in 73 F 1 progeny obtained from five crosses with cultivated sugarcane as female parents and E. fulvus as male parents. Three random 10mers were used as primers in random amplified polymorphic DNA (RAPD) reactions. A total of 10 E. fulvus –specific RAPD markers were cloned and sequenced, of which four were successfully applied as sequence‐characterized amplified region (SCAR) markers. Based on the four RAPD marker sequences, five SCAR primer pairs were designed for polymerase chain reaction amplifications. Of the 73 progeny examined, 58 produced E. fulvus –specific SCAR bands, thus representing true hybrids of cultivated sugarcane and E. fulvus These results demonstrate the first use of SCAR marker analysis as an effective tool for the identification of intergeneric sugarcane hybrids.