Premium
Validation of Low‐Phytate QTLs and Evaluation of Seedling Emergence of Low‐Phytate Soybeans
Author(s) -
Gao Y.,
Biyashev R.M.,
Maroof M.A. Saghai,
Glover N.M.,
Tucker D.M.,
Buss G.R.
Publication year - 2008
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2007.11.0633
Subject(s) - biology , quantitative trait locus , seedling , genetics , backcrossing , population , marker assisted selection , genotype , transgressive segregation , mutant , glycine , genetic linkage , gene , horticulture , amino acid , demography , sociology
M153‐1‐4 is a low‐phytate (LP) soybean [ Glycine max (L.) Merr.] mutant derived from chemical mutagenesis, possibly from a single gene mutation. However, the inheritance of LP in CX1834, as descended from M153‐1‐4, has been shown to involve two independent loci which were subsequently mapped to linkage groups (LGs) L and N. The objectives of this study were to (i) validate the two previously identified LP QTLs in CX1834 using 208 F 2:5 lines of CX1834‐1‐6 × V99‐3337, (ii) characterize associations of LP QTLs with phytate content in a set of F 2:5 and backcross (BC) lines as derived from phenotypic or genotypic selections, and (iii) evaluate the impact of LP on seedling emergence. Overall, the previously reported LP QTLs were validated in this study. However, observations of natural genetic variation and transgressive segregants suggest that additional LP QTLs could exist, potentially contributed by V99‐3337, the other parent of the validation population. A perfect correlation was found between phenotypic (phytate‐based) and genotypic (marker‐based) selections for LP lines in this study. However, findings of LP marker genotypes in the high phytate (HP) phenotypic selections suggest a potential linkage between HP and LP genes on LG L in CX1834. LP lines did not have uniformly low emergence in this study. Under some environments, HP and LP lines had little difference in seedling emergence in the field.