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Antioxidant Responses of Creeping Bentgrass Roots to Waterlogging
Author(s) -
Wang Kehua,
Jiang Yiwei
Publication year - 2007
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2006.07.0498
Subject(s) - antioxidant , superoxide dismutase , malondialdehyde , biology , peroxidase , glutathione reductase , agrostis , cultivar , glutathione peroxidase , waterlogging (archaeology) , botany , biochemistry , enzyme , poaceae , ecology , wetland
Antioxidant enzymes protect plant cells from oxidative injury induced by hypoxia. The objective of this study was to identify the responses of antioxidant enzymes to different depths of waterlogging (WL) in creeping bentgrass ( Agrostis stolonifera L.) roots. ‘Penncross’ and ‘G‐6’ were subjected to 21 d of WL at 1 cm (WL‐1) and 15 cm (WL‐15) below the soil surface, respectively. The turf quality of Penncross was not acceptable under both WL conditions, while G‐6 had an acceptable quality of 6.5 under WL‐15. The activity of superoxide dismutase (SOD) increased by 32 and 26% for Penncross and 83 and 44% for G‐6 under WL‐15 and WL‐1, respectively. One isoform of Mn‐SOD and three isoforms of Cu/Zn‐SOD were identified in both cultivars under all treatments. The activity of ascorbate peroxidase (AP) significantly decreased by 46 and 40% for Penncross, while AP activity decreased 25% and increased 9% for G‐6 under WL‐1 and WL‐15, respectively. One isoform of AP was identified with strong intensity in both cultivars under all treatments. The content of hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA, 1,1,3,3‐tetramethoxypropane) and the activities of glutathione reductase (GR) and peroxidase (POD) were not affected by WL for both cultivars. These results indicated that SOD and AP were mainly involved in WL‐induced antioxidant responses, and the partial WL could also significantly affect root antioxidant activities, particularly in WL‐sensitive cultivar.