Registration of Slow Darkening Pinto Bean Germplasm Line SDIP‐1

Pinto bean (Phaseolus vulgaris L.) germplasm line SDIP-1 (Reg. no. GP-260, PI 642703) was developed by the dry bean breeding program at the University of Idaho, Kimberly Research and Extension Center in 2003. Germplasm line SDIP-1 was released in 2006 for its unique slow darkening, aging, or oxidizing pinto seed coat color, a highly sought after trait not found in the North American cultivars developed thus far. Pinto SDIP-1 breeding line was derived from the doublecross ‘Kodiak’/‘Poncho’//‘Matterhorn’/BelDakMiRMR-14 made in 1999–2000. Pinto Kodiak and great northern Matterhorn were released by the Michigan Agricultural Experiment Station in 1998–1999 (Kelly et al., 1999a,b). Both cultivars have an indeterminate upright or erect Type II growth habit with small to medium vine (Singh, 1982) in southern Idaho. Both carry I gene resistance to US-6 and all other strains of Bean common mosaic virus (BCMV). In addition, Kodiak exhibits local necrosis or pinpoint lesions when inoculated with the NL-3K strain of Bean common mosaic necrosis virus (BCMNV). In contrast, NL-3K causes top-necrosis and eventual plant death of Matterhorn. Kodiak and Matterhorn are resistant (no disease symptoms) to race 38 (Andean) of Uromyces appendiculatus (Pers.) Ung. (the cause of bean rust) and carry the Ur-3 gene exhibiting small pustules when inoculated with race 53 (Middle American) of the pathogen. Pinto Poncho is a ProVita cultivar with Type III growth habit and is resistant to the NY15 strain of BCMV. But, Poncho is susceptible to the US-6 strain of BCMV, NL-3K strain of BCMNV, and race 53 ofU. appendiculatus. Stavely et al. (1998) at the USDA-ARS, Beltsville, Maryland, and North Dakota and Michigan Agricultural Experiment Stations cooperatively developed pinto germplasm line BelDakMiRMR-14. BelDakMiRMR-14 has Type III growth habit and pyramided resistance to all known strains or races of BCMV, BCMNV, and U. appendiculatus in the U.S. The double-cross F2 population was grown in the field at Parma. The F2–derived F3 (F2:3) progeny-row was grown in the greenhouse where a single-plant selection was made. The F3:4 progeny-row was grown in the field at Kimberly and all selected plants were harvested in bulk. A single-plant F5 selection was made in the field at Parma. The F6 progeny-row was grown in the greenhouse, all plants harvested in bulk, followed by seed increase in the field at Kimberly. SDIP-1 was tested in the Idaho Dry Bean Trial (IDBT), Western Regional Bean Trial (WRBT), and/or Cooperative Dry Bean Nursery (CDBN) as UIP6–72P-9G and ABL 9 in 2004 and 2005. SDIP1 also was evaluated for major bacterial, fungal, and viral diseases in separate greenhouse nurseries at Kimberly and Filer, Idaho from 2003 to 2006. After 8 mo of storage at room temperature at Kimberly SDIP-1 had a seed coat darkening score of 2 on a 1 to 9 scale, where 1 5 no appreciable darkening after harvest, and 9 5 completely darkened seed coat. In contrast, ‘Bill Z’ scored 5, ‘Othello’ 6, ‘Buster’ 7, and Kodiak and UI 320 scored 9. Pinto germplasm line SDIP-1 has an indeterminate semi-prostrate Type III growth habit with small vine. In the IDBT, across 10 locations, in 2005, pinto SDIP-1 averaged 2061 kg ha compared to 2365 kg ha for Othello, 2445 kg ha for Bill Z, and 2462 kg ha for Buster. SDIP-1 had a mean 100 seed weight of 37.5 g and matured in 94 d. The seed weight and maturity of Othello, Bill Z, and Buster were, respectively, 37 g and 84 d, 35 g and 93 d, and 40 g and 93 d. SDIP-1 carries a recessive resistance to BCMV (strains NY-15 and US-6) and BCMNV (strain NL-3K), but it is susceptible to anthracnose [caused by Colletotrichum lindemuthianum (Sacc. & Magnus) Lams.Scrib. races 23 and 73], common bacterial blight [caused by Xanthomonas campestris pv. phaseoli (Smith) Dye], halo blight [caused by Pseudomonas syringae pv. phaseolicola (Burkh.) race 2], rust [caused by U. appendiculatus races 38 and 53], and white mold [caused by Sclerotinia sclerotiorum (Lib) de Bary]. A small quantity of seed of SDIP-1 for research purposes is available from S. Singh for the first 5 yr. Recipients of seed are asked to make appropriate recognition of the source of germplasm if it is used in the development of a new cultivar, germplasm, parental line or genetic stock.