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Transgenic Expression of Onion Leaf Lectin Gene in Indian Mustard Offers Protection against Aphid Colonization
Author(s) -
Hossain Munshi Azad,
Maiti Mrinal K.,
Basu Asitava,
Sen Supriya,
Ghosh Arnab K.,
Sen Soumitra K.
Publication year - 2006
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2005.11.0418
Subject(s) - biology , lipaphis erysimi , mustard plant , lectin , brassica , allium sativum , aphid , allium , agglutinin , genetically modified crops , botany , transgene , gene , biochemistry
Genes of three naturally occurring monocot mannose‐binding lectins from snowdrop ( Galanthus nivalis L. agglutinin, GNA), garlic ( Allium sativum L. leaf agglutinin, ASAL), and onion ( Allium cepa L. agglutinin, ACA) and a recombinant fusion lectin between ASAL and ACA genes were expressed in a bacterial system. The pure and active form of the recombinant lectin peptides were utilized for estimation of their sensitivity potential against feeding nymphs of mustard aphid [ Lipaphis erysimi (Kaltenbach)], a major sap‐sucking insect pest of Indian mustard [ Brassica juncea (L.) Czern.], an oilseed crop. The artificial diet bioassay revealed that ACA and the fusion lectin contained higher toxicity potential than GNA and ASAL. Ectopic expression of these lectins in mustard plants confirmed their protective capacity on the development of the population of aphids on transgenic plants. Based on the strong possibilities that lectins originating from diverse sources would have differential insecticidal potential against different insects, deployment of the appropriate lectin gene figures as crucial in the transgenic approach to protect crop plants against sap‐sucking insect pests.