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Introgression of Perennial Teosinte Genome into Maize and Identification of Genomic In Situ Hybridization and Microsatellite Markers
Author(s) -
Tang Qilin,
Rong Tingzhao,
Song Yunchun,
Yang Junpin,
Pan Guangtang,
Li Wanchen,
Huang Yubi,
Cao Moju
Publication year - 2005
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2005.0717
Subject(s) - biology , introgression , backcrossing , perennial plant , hybrid , microsatellite , chromosome , genetics , botany , gene , allele
To transfer desirable characters from perennial teosinte [ Zea perennis (Hitchc.) Reeves & Mangelsd.] into maize ( Z. mays L.), we have generated an interspecific hybrid and its backcross generations (BC 1 F 3 ). The maize × perennial teosinte BC 1 F 3 (MZI202) resembled maize, with chromosome number 2 n = 20. MZI202 was studied by multicolor genomic in situ hybridization (McGISH) and simple‐sequence repeat (SSR) microsatellites markers. The McGISH experiments provided direct evidence that MZI202 was a maize–perennial teosinte substitution line with introgression of three alien chromosomes from perennial teosinte. The SSR assay further confirmed that a single chromosome 6 and the pair of chromosome 10 of maize were replaced by perennial teosinte chromosomes in MZI202.