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Expanding the Pool of PCR‐Based Markers for Oat
Author(s) -
Jannink J.-L.,
Gardner S. W.
Publication year - 2005
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2005.0285
Subject(s) - biology , avena , polymerase chain reaction , primer (cosmetics) , restriction fragment length polymorphism , genetics , genetic marker , population , computational biology , botany , gene , chemistry , demography , organic chemistry , sociology
Polymerase chain reaction (PCR)–based markers are generally more rapid and less expensive to assay than hybridization‐based markers (e.g., restriction fragment length polymorphisms [RFLP]), making them useful for breeding applications, but few of such markers are available for oat ( Avena sativa L.). Approaches to develop new markers cheaply include using primer pairs designed for other species or using publicly available sequence information. In this study we report on the design of 32 markers using publicly available oat sequence data and on the map locations of 20 loci from 16 markers on the oat ‘Ogle’ × ‘TAM O‐301’ population.