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Engineering Higher Yield and Herbicide Resistance in Rice by Agrobacterium ‐Mediated Multiple Gene Transformation
Author(s) -
Cao M. X.,
Huang J. Q.,
Wei Z. M.,
Yao Q. H.,
Wan C. Z.,
Lu J. A.
Publication year - 2004
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2004.2206
Subject(s) - biology , transgene , genetically modified rice , transformation (genetics) , agrobacterium , genetically modified crops , oryza sativa , panicle , agrobacterium tumefaciens , gene , southern blot , cultivar , genetics , microbiology and biotechnology , botany
The Vitreoscilla hemoglobin gene ( VHb ), trans ‐zeatin secretion gene ( tzs ), and the modified 5‐enolpyruvylshikimate‐3‐phosphate synthase gene ( EPSPS ), as linked expression cassettes, were simultaneously introduced into immature embryos of the rice ( Oryza sativa L.) cultivars Xiushui‐11, Qiufeng, Youfeng, and Hanfeng by Agrobacterium tumefaciens A total of 1153 transgenic lines composed of 4222 plants were obtained through selection for hygromycin (hyg) B resistance. Genomic polymerase chain reaction (PCR), southern and northern blotting analyses, and other relative tests showed that all transgenes had been integrated into the rice genome and expressed effectively. Approximately 90.2% of the transgenic lines harbored all the transgenes. Expression analysis revealed that all transgenes coexpressed stably in transgenic plants, and the frequency of coexpression was about 85%. Statistically significant increases were observed in plant height, panicle length, total grains per panicle, and filled grains per panicle in transgenic plant lines compared with the control. Our study demonstrates a possible way to introduce different transgenes as linked expression cassettes within a single vector into the plant genome. Moreover, this transgenic approach has great potential in developing new rice cultivars with increased productivity and enhanced tolerance to the herbicide glyphosate.